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Malay - Platform
- Leica Bond-PRIME
- Clone/manufacturer
- 3E2C1 (Invitrogen)
- Antibody dilution
- 1:400
- Antibody incubation time/temp
- 15 mins at 37°C
- Antigen retrieval buffer time/temp
- Leica ER2 40 mins at 100°C
- Amplification
- Not applicable
- Detection kit
- DS284 DAB Polymer Refine
- Program (mark)
- General 2025 (4.5/5)
Claudin-4 (Clostridium perfringens enterotoxin receptor) is a tight junction protein encoded by the gene CLDN4. Claudin-4 has been shown to distinguish adenocarcinoma from malignant mesothelioma with 99% specificity in malignant effusions (1). Basal-like tumours also demonstrated overexpression of Claudin-4 (2). Loss of Claudin-4 was also seen in 69% of advanced gastric cancers and correlated with poor differentiation (3).
Recommended Controls
The criteria for acceptable staining is membranous. The mesothelioma should be negative, normal lung and lung adenocarcinoma should demonstrate positive staining. The recommended controls to use is a positive (lung adenocarcinoma) and negative tumour (mesothelioma).
Expected staining pattern
Mesothelioma
Lung adenocarcinoma
Normal lung 1
Normal lung 2
Disclaimer
These methods are intended as a guide only. Laboratories that wish to implement these methods should perform internal validation/verification prior to use. The RCPAQAP does not make any claim or warranty for the accuracy or performance of these methods.
References
Jo VY, Cibas ES, Pinkus GS. Claudin-4 immunohistochemistry is highly effective in distinguishing adenocarcinoma from malignant mesothelioma in effusion cytology. Cancer Cytopathol. 2014 Apr;122(4):299-306.
Lanigan F, et al. Increased claudin-4 expression is associated with poor prognosis and high tumour grade in breast cancer. Int J Cancer. 2009 May 1;124(9):2088-97.
Kolokytha P, et al. Claudin-3 and claudin-4: distinct prognostic significance in triple-negative and luminal breast cancer. Appl Immunohistochem Mol Morphol. 2014;22(2):125-31.
Images of mesothelioma, lung adenocarcinoma and normal lung_ QAP survey results