ERG (Erythroblast Transformation-specific related Gene)

ERG is a nuclear transcription factor encoded on chromosome 21q22, and is a highly sensitive marker of vascular differentiation. It shows strong nuclear staining in normal endothelial cells and in benign and malignant vascular tumors (such as hemangioma, angiosarcoma, and Kaposi sarcoma). ERG is also positive in tumours with ERG gene rearrangements, including about 50% of prostatic adenocarcinomas and a subset of Ewing sarcomas, as well as in some cases of acute myeloid leukemia / myeloid sarcoma. ERG is used to confirm endothelial lineage and to help identify ERG‑rearranged tumours (1).

Recommended Controls

Criteria for acceptable staining is a nuclear staining pattern. Recommended control to include a vascular haemangioma, tonsil or appendix. Positive nuclear staining in the haemanagioma as well as in the endothelial cells of vessels in the tonsil. Appendix should have no staining.

Expected staining pattern

Haemangioma

Tonsil

Disclaimer

These methods are intended as a guide only. Laboratories that wish to implement these methods should perform internal validation/verification prior to use. The RCPAQAP does not make any claim or warranty for the accuracy or performance of these methods.