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Malay - Platform
- Leica Bond X
- Clone/manufacturer
- Polyclonal (B0471-Dako Agilent)
- Antibody dilution
- 1:500
- Antibody incubation time/temp
- 30 mins at ambient room temperature
- Antigen retrieval buffer time/temp
- Microwave EDTA 20 mins at 100°C
- Amplification
- Not applicable
- Detection kit
- DS9800 DAB Polymer Refine
- Program (mark)
- General 2015 (5/5)
The Helicobacter pylori immunohistochemistry stain is used to detect the small gram‑negative, spiral or curved shaped bacteria that colonise the gastric mucosal surface. These organisms sit within the mucus layer near the gastric pits, making them easy to miss on the H&E stain when they are low in numbers. IHC is therefore a more sensitive method to confirm infection, especially in cases with chronic active gastritis, where neutrophils and plasma cells form a superficial inflammatory band. The classic histologic pattern of H. pylori gastritis. The IHC is used to help identify organisms in biopsies particularly in patients or cases with low organism numbers (1-4).
Recommended Controls
Criteria for acceptable staining in a H. pylori positive gastric tissue, apical staining of the cell membranes of the infected gastric epithelium and staining of the bacilli in the gastric lumen above the epithelium.
Expected staining pattern
H. Pylori infected gastric sleeve
Disclaimer
These methods are intended as a guide only. Laboratories that wish to implement these methods should perform internal validation/verification prior to use. The RCPAQAP does not make any claim or warranty for the accuracy or performance of these methods.
References
Storozuk T, Setia N. Helicobacter pylori. PathologyOutlines.com website. https://www.pathologyoutlines.com/topic/stomachhelicobacter.html.
Lott R.L and Brown R.W. Histological Preparations. Common problems and their solutions. CAP 2009 ISBN: 978-0-930304-95-9. 65-75.
Riba AK, Ingeneri TJ, Strand CL. Improved Histologic Identification of Helicobacter pylori by Immunohistochemistry Using a New Novocastra Monoclonal Antibody. Lab Med. 2011; 42(1):35-39.
Leong A.S-Y, Cooper K and Leong F.J. W-M. Manual of Diagnostic Antibodies for Immunohistology, 2nd edition. Greenwich Medical Media. ISBN: 1 84110 100 1. 2003. 253-254.
Image of H. Pylori infected gastric sleeve_QAP survey results