OCT3/4

OCT3/4 (Octamer transcription factor 3/4) has been proposed as a useful marker for germ cell tumors which exhibit features of pluripotentiality, including seminoma/dysgerminoma/germinoma and embryonal carcinoma, and establishing a germ cell origin for some metastatic tumors of uncertain primary origin (1).

Recommended Controls

Criteria for acceptable staining is nuclear.The recommended positive control to use for a OCT3/4 stain is to always include a positive germ cell tumour as well as a section of appendix, which will be the negative control.

Appendix

Seminoma

Disclaimer

These methods are intended as a guide only. Laboratories that wish to implement these methods should perform internal validation/verification prior to use. The RCPAQAP does not make any claim or warranty for the accuracy or performance of these methods.