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Malay - Platform
- Roche Ventana Benchmark Ultra
- Clone/manufacturer
- BC34 (ACI3099C - Biocare Medical)
- Antibody dilution
- 1:40
- Antibody incubation time/temp
- 32 mins at 37°C
- Antigen retrieval buffer time/temp
- Ventana CC1 60 mins at 100°C
- Amplification
- Not applicable
- Detection kit
- 760-501 Ventana OptiView
- Program (mark)
- General 2024 (5/5)
SOX10 (SRY- box containing gene 10), a transcription factor is expressed in schwannian and melanocytic neoplasms (1). It is also consistently expressed in Schwann cell tumours, gastric tract and metastatic melanomas (2). Interpretation can be difficult due to melanin pigment (3). A higher SOX10 expression may be associated with more aggressive tumour characteristics in Triple Negative Breast Cancer (4).
Recommended Controls
The criteria for acceptable staining is nuclear. The recommended positive control to use for a SOX10 stain is to always include a positive melanoma tumour as well as a section of normal skin and/or appendix.
Malignant melanoma
Normal skin
Disclaimer
These methods are intended as a guide only. Laboratories that wish to implement these methods should perform internal validation/verification prior to use. The RCPAQAP does not make any claim or warranty for the accuracy or performance of these methods.
References
Biocare Medical SOX10 specification sheet- BC34 clone- http://biocare.net/wp-content/uploads/3099.pdf
Sox10 – A positive marker for most melanomas. Am J Sug Pathology 2015;39:826
Pathology Outlines
Clinicopathological significance of SOX10 expression in TNBC
Images of melanoma and normal skin_ QAP survey result