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Malay - Platform
- Leica Bond III
- Clone/manufacturer
- WT-49 (PA0562 - Leica VisionBioSystems)
- Antibody dilution
- Predilute
- Antibody incubation time/temp
- 30 mins at ambient room temperature
- Antigen retrieval buffer time/temp
- Leica ER2 30 mins at 100°C
- Amplification
- Not applicable
- Detection kit
- DS9800 DAB Polymer Refine
- Program (mark)
- General 2016 (4.5/5)
Wilms Tumor (WT), a sporadic and familial childhood kidney tumor, is genetically heterogeneous. Wilms tumor is associated with mutations of WT1, The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilms tumor and mesothelioma (1-2).
Recommended Controls
The criteria for acceptable staining is nuclear. The recommended controls to include is fallopian tube, kidney and ovarian serous carcinoma or prostate cancer. Fallopian tube will show strong staining in all epithelial and smooth muscle cells; kidney will show nuclear staining in podocytes while the epithelial cells of the tubules will be negative. Ovarian serous carcinoma is a strong expressor while the prostate cancer will show no staining in the tumour cells.
Serous adenocarcinaoma
Disclaimer
These methods are intended as a guide only. Laboratories that wish to implement these methods should perform internal validation/verification prior to use. The RCPAQAP does not make any claim or warranty for the accuracy or performance of these methods.
References
ThermoScientific Specification data sheet https://static.thermoscientific.com/images/D12357~.pdf
Leong A.S-Y, Cooper K and Leong F.J. W-M. Manual of Diagnostic Antibodies for Immunohistology, 2nd edition. Greenwich Medical Media. ISBN: 1 84110 100 1. 1999. 447-448.
Image of ovarian serous tumour_QAP survey.